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Detail Research
Research Title Platelet Alloantibodies and platelet transfusion response in Multitransfused hematology platelet at Cipto Mangunkusumo Hospital
Focus Infectious Diseases
Investigator Anna Mira Lubis
NIM
Adviser -
Category Clinic
Research Date (Start Date) 00 Januari 2008
Exam Date 00 00 2008
Keywords
Abstract

Backgrounds:

Random donor multitransfusion and non-leukocyte depleted blood product have been known as a risk factors platelet alloimmunization (HLA and HPA) and remains a big problem due to platelet transfusion refractoriness. Need further investigation regarding transfusion response and alloantibodyi n multitransfusedin Indonesia, to serve a better transfusion by performing donor selection afterwards.

 

Objectives:

To found : (l) platelet transfusion response by CCI I hour post transfusion measurement, (2) platelet alloantibody detection (Ig G) to HLA class l, epitope GP IIb/IIIa, Ib/IX and IalIIa antigens by Indirect ELISA, (3) association between platelet alloantibody and platelet transfusion response in multitransfused hematology patients.

 

Methods:

Observasional study was conducted to transfusion given to multitransfused hematology patients, excluded for conditions and comorbidities that could influenced CCI 1

hour post transfusion, during March to June 2008, at Cipto Mangunkusumo Hospital, with sample size estimation of 36.CCI was measured within 1 hour post transfusion with cut off level 7500 m2/ml. Platelet alloantibody detected by Indirect ELISA using ccmercial kit Pak-z LE. Statistical analysis were performed using chi-square and logistic regression with Cl 95%.

 

 

Main Results:

Thirty six transfusion included in the study with 2l participants. As much as 33%

failed to achief satisfactory response with CCI < 7500. HLA class I antibody was detected in 38,9% transfusion and 2,8% alloimmunized subject was positif to epitope GP IIb/IIIa. There is an association between Ig G HLA class l and platelet transfusion failure (PR 4,7, p = 0,003, CI 95% 1,535-14,47 with Adjusted PR 11,4).

 

Conclusion:

Patients with HLA class I antibody tend to came with platelet transfusion failure. The association cpitope CP antibody with platelet transfusion response needs further investigation.

Key words:

platelet, transfusion, alloantibodies