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Research Title The Effect of Extemely Low Frequency- Electromagnetic Field Exposure to Number and Morphology of Follicle de Graaf of Mice and Inter-generational Cumulative Effect
Focus Others (-)
Investigator Sovina Helyati
NIM 0906552725
Adviser Puji Sari
Category Biomedic
Research Date (Start Date) 01 Desember 2012
Exam Date 21 Juli 2013
Keywords atresia follicle de Graaf ; cumulative effect; ELF-EMF, follicle de Graaf; generation
Abstract

Extremely low frequency- electromagnetic fields are categorized as non-ionising radiation which could be produced by electrical household appliances or communication devices. ELF-EMF long term exposure suggested can disturb releasing gonadotropine hormon (GnRH) which have a role in follicle development and maturation, especially pre antral follicle development. Therefore, this study aims to evaluate the influence  of extremely low frequency-electromagnetic field in varying frequencies on number and morphology of follicle de Graaf. This study also evaluate ELF-EMF cumulative effect on multiple generation (F1, F2, and F3). Twelve pairs  mice strains Swiss Webster as parental mice (F0)  were divided into four groups. One group as control and three other groups were exposed by magnetic field,which doses were 3kV 5,5µT, 4kV 5,4µT,and  5kV 5,3µT. These treatments were also given to first, second, and third generation.  Five female mices aged 2,5 months from each first (F1), second (F2),and third (F3) generation on each groups were examined to evaluate number and morphology (atresia follicle) of de Graaf follicle from their ovarium. The hypothesis was tested using Kruskall-Wallis which show no significant difference between groups on follicle de Graaf number (p=0,480) and atresia follicle de Graaf (p=0,336). These results suggest ELF-EMF exposure don't affect number and morphology of follicle de Graaf and there are no cumulative effect on multiple generation.