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Detail Research
Research Title Activity of Lactate Dehydrogenase in Cardiac Tissue of Systemic Hypoxia Induced Rats
Focus Others (-)
Investigator Faathimah Mahmudi Isma'il
NIM 0906508030
Adviser Ani Retno Prijanti
Category Biomedic
Research Date (Start Date) 01 Maret 2011
Exam Date 20 Juli 2013
Keywords Anaerobic glycolysis, Heart, Hypoxia, Lactate dehydrogenase (LDH).

Hypoxia is a state of oxygen deficiency in tissues or inability to use oxygen. This condition has been associated with the pathology of the leading causes of death, including cardiovascular disease. In order to maintain the ability to produce ATP although oxygen is limited, the cell has an ability to change from aerobic to anaerobic glycolysis. This research aims to evaluate the lactate dehydrogenase (LDH) spesific activity during systemic hypoxia in the heart. This research is an experimental study on 25 Sprague-Dawley rats that randomly divided into one group of normoxic rats as control and four groups of hypoxia induced rats (10% O2 and 90% N2) for 1 day, 3 day, 7 day, and 14 day, so that each group consisted of 5 rats. Measurement of LDH specific activity was done using LDH QuantiChromTM (DLDH-100) kit. Cardiac LDH specific activity of hypoxia induced rat tend to decrease during hypoxia compared with normoxic rat. Analysis with Kruskal-Wallis shows that there is no significant difference of cardiac LDH specific activity between groups (p = 0.391). So, it can be concluded that there is no difference of cardiac LDH activity between normoxic and systemic hypoxia induced rats.