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Research Title FIELD EVALUATION OF BIO-RAD NS1 AG STRIP AS DIAGNOSTIC KIT FOR PRIMARY AND SECONDARY DENGUE INFECTION IN JAKARTA
Focus Infectious Diseases
Investigator Jusica Putri
NIM 1006718031
Adviser Beti Ernawati Dewi
Category Clinic
Research Date (Start Date) 07 November 2010
Exam Date 04 Agustus 2013
Keywords Bio-Rad; Dengue; NS1; Primary; Secondary; Sensitivity; Specificity
Abstract

Dengue Infection has been one of the serious health problems in Indonesia. Non Structural-1 (NS-1) antigen was known to be the early diagnostic indicator of dengue infection. The purpose of this research is identifying the sensitivity and specificity of a diagnostic kit, called Biorad NS-1 Ag Rapid Test, which use NS-1 Antigen as the marker to detect primary and secondary dengue infection. Cohort with consecutive sampling was the most suitable research design to be applied in this study. We used Reverse Transcription-Polymerase Chain Reaction (RT-PCR), or virus isolation in C6/36 cell line or the increment of antibody titer as the gold standard in this study. We also used SPSS 17.0 with tables 2x2 and area under the curve (AUC) of the Receiver Operating Curve to analyze the data. Population data in this research were 102 patients and 68.3% of them confirmed as dengue infection patient. Furthermore, the positive results are classified into primary and secondary infection, which are 27 (39.7%) and 35 (51.5%) people, respectively. The sensitivity and specificity of Bio-Rad NS1 Ag Strip in primary dengue infection were 96.30% and 100%, respectively. However, the sensitivity and specificity of Bio-Rad NS1 Ag Strip for secondary dengue infection were 85.71% and 100 %, respectively. Bio-Rad NS1 Ag Strip has high sensitivity and specificity to determine both primary and secondary infection. As the result, Bio-Rad NS1 Ag Strip is recommended to be used for early detection of primary and secondary dengue infection.